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Laboratory Experiments in Microbiology (8th Edition) - Ted R. Johnson, Spiral-bound
Benjamin Cummings
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Release Date
2/20/2006
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ISBN-13
9780805382921 | 978-0-8053-8292-1
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ISBN
0805382925 | 0-8053-8292-5
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Format
Spiral-bound
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Author(s)
Ted R. Johnson
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Newly revised to correspond to all current undergraduate one-semester microbiology textbooks. This lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology and is an ideal companion to Microbiology: An Introduction, Ninth Edition by Tortora, Funke, and Case. Microscopy: Use and Care of the Microscope, Examination of Living Microorganisms. Staining Methods, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining, Structural Stains (Endospore, Capsule, Flagella), Morphologic Unknown. Cultivation of Bacteria: Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria. Microbial Metabolism: Carbohydrate Catabolism, Fermentation, Protein Catabolism, Respiration, Rapid Identification Methods. Microbial Growth: Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms. Control of Microbial Growth: Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing. Microbial Genetics: Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting, Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens. The Microbial World: Unknown Identification and Bergey's Manual, Fungi: Yeasts, Fungi: Molds, Phototrophs: Algae and Cyanobacteria, Protozoa, VIRUSES, Isolation and Titration of Bacteriophages, Plant Viruses. Interaction of Microbe and Host: Epidemiology, Koch's Postulate, IMMUNOLOGY, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique. Microorganisms and Disease: Bacteria of the Skin, Bacteria of the Respiratory Tract, Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Urogenital Tract, Identification of an Unknown from a Clinical Sample. Microbiology and the Environment: Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique, Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles, Microbes in Soil: Bioremediaton. Appendices: Pipetting, Dilution Techniques and Calculations, Use of the Spectrophotometer, Graphing, Use of the Dissecting Membrane, Use of the Membrane Filter, Electrophoresis, Keys to Bacteria. For all readers interested in microbiology.
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